Apache2 - WSGI file not found Issue - apache2

Question

I run bash script file through python code. The script is located /home/myscript.sh. The script convert html to pdf and print. If I run the python program in development mode it works fine but if I run in WSGI production mode it give me error "File not found" to print. But if I give access 7777 to home directory it works fine. And I need to do this access step every time whenever machine restarted. Also it not good solution.
I search a lot and change apache2.conf file
<Directory /home/>
Options Indexes FollowSymLinks
AllowOverride None
Require all granted
</Directory>
But still there is problem. Please help me. I used Apache 2.4.9 version. Advance thanks
Update
In /etc/apache2/apache2.conf file
"User ${APACHE_RUN_USER}" and "Group ${APACHE_RUN_GROUP}"
And in envvars it sets like
export APACHE_RUN_USER=www-data
export APACHE_RUN_GROUP=www-data
Still problem exist.

Answer 1

If issue is with directory
Example /home
Move the file from /home to /var/www
sudo chown www-data:www-data /var/www/filename
or
sudo chown www-data:www-data /var/www/filename
If the issue with USB port
Example
ttyUSB0
,
ttyACM0
,
ttyACM1
Write script to change the owner
#!/bin/bash
sudo chown www-data:www-data /dev/ttyACM0
Call this script from your code using subprocess.call
But allow the user www-data to run this file as sudo with out password
sudo visudo
and add the below line in the last
www-data ALL = (ALL) NOPASSWD : /var/www/filename
or
www-data ALL = (ALL) NOPASSWD : ALL